Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Susceptibility Analysis in Several Mouse Strains Reveals Robust T-Cell Responses After Mycoplasma pneumoniae Infection in DBA/2 Mice

doi: 10.3389/fcimb.2020.602453

Figure Lengend Snippet: Mycoplasma pneumoniae bacterial load in bronchoalveolar lavage fluid after infection in several inbred mouse strains. Mice were infected with Mycoplasma pneumoniae (Mp) intranasally. (A) On day 1 after infection, the Mp bacterial load in bronchoalveolar lavage fluid (BALF) was determined using real-time PCR. (B) On days 1, 3, and 5 post-infection, the Mp bacterial load in BALF obtained from BALB/c and DBA/2 mice was determined by real-time PCR. (C) On day 1 post-infection, Mp bacterial load in BALF from BALB/c and DBA/2 mice was analyzed by bacterial culture assay. (D) On days 1 and 3 post-infection, the level of lactate dehydrogenase activity in BALF was determined. Data are shown as means ± SD. Each experiment was performed twice. (A) n = 4 or 5 per group, (B–D) n = 5 or 6 per group. (A) * P < 0.05; ** P < 0.01 as indicated by Tukey’s test. (B, C) * P < 0.05; ** P < 0.01 as indicated by Student’s t -test.

Article Snippet: In this study, 6- to 7-week-old, male A/J, BALB/c, C3H/HeJ, C3H/HeN, C57BL/6J, CBA/N, DBA/1, and DBA/2 mice were purchased from SLC (Hamamatsu, Japan).

Techniques: Infection, Real-time Polymerase Chain Reaction, Activity Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Susceptibility Analysis in Several Mouse Strains Reveals Robust T-Cell Responses After Mycoplasma pneumoniae Infection in DBA/2 Mice

doi: 10.3389/fcimb.2020.602453

Figure Lengend Snippet: Immune cell numbers in bronchoalveolar lavage fluid after Mycoplasma pneumoniae infection in several inbred mouse strains. (A) Mice from several strains were infected intranasally with Mycoplasma pneumoniae . On day 1 post-infection, the numbers of CD45 + immune cells, neutrophils, and alveolar macrophages in bronchoalveolar lavage fluid (BALF) were determined using flow cytometry. (B) The numbers of CD45 + immune cells, neutrophils, and alveolar macrophages in BALF from naive BALB/c mice and naive DBA/2 mice were determined by flow cytometry. Data are shown as means ± SD. Each experiment was performed twice. (A, B) n = 5 per group. (A) * P < 0.05; ** P < 0.01 as indicated by Tukey’s test.

Article Snippet: In this study, 6- to 7-week-old, male A/J, BALB/c, C3H/HeJ, C3H/HeN, C57BL/6J, CBA/N, DBA/1, and DBA/2 mice were purchased from SLC (Hamamatsu, Japan).

Techniques: Infection, Flow Cytometry

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Susceptibility Analysis in Several Mouse Strains Reveals Robust T-Cell Responses After Mycoplasma pneumoniae Infection in DBA/2 Mice

doi: 10.3389/fcimb.2020.602453

Figure Lengend Snippet: Immune responses after Mycoplasma pneumoniae infection. (A) Schematic representation of the experiment. (B–D) BALB/c and DBA/2 mice were infected intranasally with Mycoplasma pneumoniae (Mp). (B, C) On day 14 post-infection, the levels of (B) Mp-specific IgG in 32-, 160-, and 800-fold diluted plasma, and (C) recombinant P1-specific IgG in 160-, 800-, and 4000-fold diluted plasma were determined using ELISA. (D) On day 14 post-infection, splenocytes were cultured with Mp for 3 days and the levels of IFN- γ and IL-17A in the culture supernatant were determined by ELISA. Data are shown as means ± SD. Each experiment was performed twice. (B–D) n = 5 per group. (B, C) Significant differences were observed only in the (B) 32-fold– and (C) 160-fold–diluted plasma samples. (B–D) * P < 0.05; ** P < 0.01 as indicated by Tukey’s test.

Article Snippet: In this study, 6- to 7-week-old, male A/J, BALB/c, C3H/HeJ, C3H/HeN, C57BL/6J, CBA/N, DBA/1, and DBA/2 mice were purchased from SLC (Hamamatsu, Japan).

Techniques: Infection, Recombinant, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Susceptibility Analysis in Several Mouse Strains Reveals Robust T-Cell Responses After Mycoplasma pneumoniae Infection in DBA/2 Mice

doi: 10.3389/fcimb.2020.602453

Figure Lengend Snippet: Mycoplasma pneumoniae bacterial load and immune cell numbers in bronchoalveolar lavage fluid after Mycoplasma pneumoniae reinfection in BALB/c and DBA/2 mice. (A) Schematic representation of the experiment. (B, C) BALB/c mice and DBA/2 mice were infected intranasally with Mycoplasma pneumoniae (Mp). On day 14 post-infection, mice were intranasally reinfected with Mp. On the day after reinfection, (B) Mp bacterial load and (C) the numbers of CD45 + immune cells, neutrophils, and alveolar macrophages in bronchoalveolar lavage fluid were determined by (B) real-time PCR and (C) flow cytometry. Data are shown as means ± SD. Each experiment was performed twice. (B, C) n = 5 per group. * P < 0.05; ** P < 0.01 as indicated by Student’s t -test.

Article Snippet: In this study, 6- to 7-week-old, male A/J, BALB/c, C3H/HeJ, C3H/HeN, C57BL/6J, CBA/N, DBA/1, and DBA/2 mice were purchased from SLC (Hamamatsu, Japan).

Techniques: Infection, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Susceptibility Analysis in Several Mouse Strains Reveals Robust T-Cell Responses After Mycoplasma pneumoniae Infection in DBA/2 Mice

doi: 10.3389/fcimb.2020.602453

Figure Lengend Snippet: CD4 + T cell-dependent neutrophil infiltration in reinfected mice. (A) Schematic representation of the experiment. (B) BALB/c and DBA/2 mice were infected intranasally with Mycoplasma pneumoniae (Mp). On day 13 post-infection, mice were injected intraperitoneally with anti-CD4 or isotype-control antibody. On day 14, mice were intranasally reinfected with Mp. The day after Mp reinfection, the numbers of CD45 + immune cells, neutrophils, and alveolar macrophages in bronchoalveolar lavage fluid were determined by flow cytometry. Data are shown as means ± SD. Each experiment was performed twice. n = 8–10 per group. * P < 0.05; ** P < 0.01 as indicated by Student’s t -test.

Article Snippet: In this study, 6- to 7-week-old, male A/J, BALB/c, C3H/HeJ, C3H/HeN, C57BL/6J, CBA/N, DBA/1, and DBA/2 mice were purchased from SLC (Hamamatsu, Japan).

Techniques: Infection, Injection, Flow Cytometry

Journal: Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia

Article Title: Selected 68 Ga-siderophores versus 68 Ga-colloid and 68 Ga-citrate: biodistribution and small animal imaging in mice

doi: 10.5507/bp.2014.052

Figure Lengend Snippet: Biodistribution of 68 Ga-TAFC, 68 Ga-FOXE, 68 Ga-acetate/colloid, 68 Ga-colloid and 68 Ga-citrate in normal DBA/2 mice 30 min and 90 min p.i.

Article Snippet: The studies were performed using DBA/2 mice (Anlab, Prague, Czech Republic).

Techniques: